Identifikasi Senyawa Flavonoid dari Kulit Batang Tanaman Rambai Padi (Sonneratia alba) dan Uji Aktivitasnya sebagai Antibakteri Staphylococcus aureus
Identification of Flavonoid Compounds from Stem Bark Sonneratia alba and its Antibacterial Activities against Staphylococcus aureus
DOI:
https://doi.org/10.36873/jjms.v1i2.217Keywords:
flavonoid, antibakteri, Staphylococcus aureus, Sonneratia alba, rambai padiAbstract
Penelitian ini bertujuan untuk mengetahui (1) bioaktivitas ekstrak metanol-air kulit batang tanaman rambai padi (Sonneratia alba) terhadap Staphylococcus aureus (2) konsentrasi minimal ekstrak kulit batang S. alba yang mampu menghambat dan membunuh 100% Staphylococcus aureus dan (3) jenis senyawa aktif yang terdapat pada kulit batang S. alba. Dari uji aktivitas antibakteri menggunakan metode dilusi menunjukkan bahwa ekstrak metanol:air kulit batang S. alba memiliki kemampuan dalam menghambat dan membunuh bakteri Staphylococcus aureus. Konsentrasi hambat minimum (KHM) dan konsentrasi bunuh minimum (KBM) yang diperoleh yakni 0,050 dan 0,075 % untuk ekstrak metanol:air (9:1) dan 0,50 dan 0,75% untuk ekstrak metanol:air (5:5). Hasil bioautografi dan interpretasi data UV-Vis menunjukkan bahwa terdapat 5 (lima) senyawa aktif yang berperan sebagai antibakteri pada ekstrak kulit batang S. alba yaitu 3 (tiga) senyawa flavonoid yang terdiri dari jenis flavan (katekin) dan flavonol dengan 3-OH bebas dan 2 unknown senyawa fenolik.
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