ANALISIS FLAVONOID TOTAL DAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL Syzygium aromaticum DAN Syzygium polyanthum
DOI:
https://doi.org/10.37304/jkupr.v10i2.5536Keywords:
Antioxidant, DPPH, clove leaf, salam leaf, flavonoidAbstract
Syzygium aromaticum and Syzygium polyanthum are species of the Myrtaceae family. Clove leaves and bay leaves contain active compounds, namely flavonoids. This research was conducted with the aim of knowing the total flavonoid content and antioxidant activity of the ethanol extract of clove (Syzygium aromaticum) and bay (Syzygium polyanthum) leaves. The extraction method used in this research is cold extraction in the form of maceration with the choice of solvent, namely 96% ethanol. The total flavonoid content test was carried out using the colorimetric method while the antioxidant activity test used the DPPH (1,1-diphenyl-2-picridhydrazil) method. The results of total flavonoid content of clove leaf ethanol extract and bay leaf ethanol extract were 206.447 mgQE/g and 165,658 mgQE/g.. The IC50 for clove leaves was 3.026 μg/mL and the IC50 for bay leaves was 3.555 μg/mL. The conclusion of the study was that the extract from clove leaves on total flavonoid levels and antioxidant activity tests had the highest value.
Downloads
References
Dwihastuti H. Aktivitas Antioksidan Dan Penetapan Kadar Flavonoid Dan Fenol Total Dari Sauropus Macranthus Hassk. Tugas Akhir Fak Farm Bandung [Internet]. 2019;33–5. Available from: http://localhost:8080/xmlui/handle/123456789/2735
Hapsari A. Kadar Flavonoid Total Teh Herbal Daun Benalu Cengkeh (Dendrophtoe Petandra L. Miq) Terhadap Variasi Lama Perebusan. Akrab Juara [Internet]. 2020;5(1):43–54. Available from: http://www.akrabjuara.com/index.php/akrabjuara/article/view/919
Primadiastri IZ, Wulansari ED, Suharsanti R. Perbandingan Kandungan Fenolik Total, Flavonoid Total Aktivitas Antioksidan Ekstrak Etanol Daun Jambu Bol ( Syzigium malaccense L .) Dan Daun Jambu Air Kancing ( Syzigium aqueum ). J Media Farm Indones. 2021;16(2):1671–5.
Wahyulianingsih W, Handayani S, Malik A. Penetapan Kadar Flavonoid Total Ekstrak Daun Cengkeh (Syzygium aromaticum (L.) Merr & Perry). J Fitofarmaka Indones [Internet]. 2016;3(2):188–93. Available from: https://jurnal.farmasi.umi.ac.id/index.php/fitofarmakaindo/article/view/221
Priya SH, Prakasan N, Purushothaman J. Antioxydant Activity, Phenolic Flavonoid Content and High-Performance Liquid Chromatography Profiling of Three Different Variants of Syzygium Cumini Seeds; J Intercult Ethnopharmacol [Internet]. 2017;6(1):108–14. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289079/
El-Refai AA, Sharaf AM, Azzaz NAE, El-Dengawy MM. Antioxidants and Antibacterial Activities of Bioactive Compounds of Clove (Syzygium aromaticum) and Thyme (Tymus vulgaris) Extracts. J Food Dairy Sci [Internet]. 2020;11(9):265–9. Available from: www.jfds.journals.ekb.eg
Hastuti YD, Mulangsri DAK. Perbedaan Kadar Flavonoid Total Ektrak Daun Salam (Syzygium polyanthum) Dengan Metode Refluks Dari Beberapa Jenis Pelarut Dan Aktivitas Antibakteri. J Ilmu Farm dan Farm Klin [Internet]. 2021;18(2):85–93. Available from: https://publikasiilmiah.unwahas.ac.id/index.php/Farmasi/article/view/5962
Chotimah C. Uji total flavonoid dan aktivitas aktioksidan dan ekstrak daun dan kulit batang dadap serep (Erythrina subumbrans (Hassk.) Merr.) menggunakan pelarut yang berbeda [Internet]. 2019 p. 1–22. Available from: http://etheses.uin-malang.ac.id/17774/
Molyneux P. The Use of The Stable Free Radical Diphenylpicryl-Hydrazyl (DPPH) for Estimating Anti-Oxidant Activity. Songklanakarin J Sci Technol [Internet]. 2004;26(2):211–9. Available from: https://www.researchgate.net/publication/237620105
Hidayati MD, Ersam T, Shimizu K, Fatmawati S. Antioxidant activity of Syzygium polynthum extracts. Indones J Chem. 2017;17(1):49–53.
