Uji Aktivitas Antioksidan Ekstrak Etanol 96% Herba Kalakai (Stenochlaena palustris (Burm. f.) Bedd) Dengan Metode Ferric Reducing Antioxidant Power (FRAP)
Keywords:
: Antioxidants, Kalakai, IC50, Reducing Power, FRAPAbstract
The body naturally produces endogenous antioxidants that can overcome the effects of free radicals. However, when the supply of free radicals increases, external antioxidants originating from natural ingredients are needed. One plant that can be used as a source of natural antioxidants is kalakai (Stenochlaena palustris (Burm. f) Bedd). Kalakai contains alkaloids, flavonoids, steroids, saponins, and tannins that can be antioxidants. One method used to evaluate the antioxidant content in samples is Ferric Reducing Antioxidant Power (FRAP), which measures reduction power as an indicator. The extraction method used was Ultrasound-Assisted Extraction (UAE) with 96% ethanol solvent. Then, qualitative and quantitative phytochemical screening was carried out. Antioxidant activity testing using the FRAP method was carried out with several concentrations, namely 80, 100, 120, 140, and 160 ppm, and vitamin C as a positive control. Next, the reduction power and IC50 were measured as indicators of antioxidant activity. The IC50 value was determined by calculating the regression analysis of % inhibition on the concentration of kalakai herb extract and vitamin C. In the FRAP method's antioxidant activity test, there was a color change from light yellow to Berlin blue. The 96% ethanol extract of the kalakai herb (Stenochlaena palustris (Burm. f.) Bedd) has an IC50 value of 23,380 ppm, and vitamin C has an IC50 value of 16,335 ppm, both of which can be classified as powerful antioxidants. The antioxidant activity of kelakai herb extract (Stenochlaena palustris (Burm. f.) Bedd) is influenced by the action of the phytochemical compounds contained therein. 96% ethanol extract of kalakai herb (Stenochlaena palustris (Burm. f) Bedd) has reduced power as an indicator of antioxidant activity using the FRAP method
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