Isolasi bakteri asam laktat asal feses bayi dan potensinya dalam menghambat pertumbuhan Escherichia coli

Authors

  • Nurmi Hasbi Departemen Mikrobiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Rosyunita Departemen Mikrobiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Adelia Riezka Rahim Departemen Parasitologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Rahmah Dara Ayunda Departemen Biokimia, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram, Mataram
  • Wayan Sulaksmana Sandhi Parwata Program Studi Pendidikan Dokter, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Afif Farras Program Studi Pendidikan Dokter, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Al Fikar Raihan Program Studi Pendidikan Dokter, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Muhammad Azim Billah Program Studi Pendidikan Dokter, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram

DOI:

https://doi.org/10.37304/jkupr.v12i1.12852

Keywords:

baby feses, Escherichia coli, isolation, lactic acid bacteri

Abstract

Lactic acid bacteria (LAB) play an important role in improving performance and mitigating disease because of their ability to maintain the physiological balance of the gastrointestinal tract and resistance to pathogenic bacteria. LAB is one of the bacteria found in the digestive tract. LAB must be isolated from their hosts to increase their efficiency as probiotic ingredients. This study aims to obtain local isolates of LAB a from baby feces through phenotypic identification and antibacterial test. The sample used in the research was the feces of babies aged 1-6 months that patients from Mataram University Hospital. Isolation was carried out by growing bacteria from feces using the Total Plate Count method on MRSA media plus CaCO3. Phenotypic characterization is morphology test, gram stain and biochemical tests. The results of bacterial isolation obtained 8 isolates of LAB. LAB produced a clear zone in the MRSA media, because these bacteria were able to secrete acid in the MRSA. The results of gram staining show that all isolates are gram positive bacteria. Based on the morphology of LAB had characteristic 6 isolates of cocci and 2 isolates of bacilli. All isolates was able to produce an inhibition zone against E. coli with 3 isolates in the moderate category and 5 isolates in the weak category. The best inhibition zone in the medium category is for cocci-shaped bacteria.The existence of LAB isolates from baby feces can be used as a reference source for further research on probiotics such as other antibacterial test

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References

Kumar R, Sood U, Gupta V, Singh M. Recent Advancements in the Development of Modern Probiotics for Restoring Human Gut Microbiome Dysbiosis. Indian J Microbiol. 2020;60(1):12–25. doi: 10.1007/s12088-019-00808-y.

Ooi, M. F., Mazlan, N., Foo, H. L., Loh, T. C., Rosfarizan, M., Rahim, R. A., et al. Effects of carbon and nitrogen sources on bacteriocin-inhibitory activity of postbiotic metabolites produced by Lactobacillus plantarum I-UL4. Malays J Microbiol. 2015;(January 2017). doi: 10.21161/mjm.13014

Waoo AA, Singh S, Pandey A, Kant G, Choure K, Amesho KTT, et al. Microbial exopolysaccharides in the biomedical and pharmaceutical industries. Heliyon. 2023;9(8):e18613. doi: 10.1016/j.heliyon. 2023.e18613

Casarotti SN, Carneiro BM, Todorov SD, Nero LA, Rahal P, Penna ALB. In vitro assessment of safety and probiotic potential characteristics of Lactobacillus strains isolated from water buffalo mozzarella cheese. Ann Microbiol. 2017;67(4):289–301. doi: 10.1007/s13213-017-1258-2

Jufri RF. Microbial Isolation. J La Lifesci. 2020;1(1):18–23.

Datta FU, Daki AN, Benu I, Detha AIR, Foeh NDFK, Ndaong NA. Uji aktivitas antimikroba bakteri asam laktat cairan rumen terhadap pertumbuhan Salmonella enteritidis, Bacillus cereus, Escherichia coli dan Staphylococcus aureus menggunakan metode difusi sumur agar. J Kaji Vet. 2019;66–85. doi: 10.35508/jkv.v0i0.1590

Kurnia M, Amir H, Handayani D. Isolasi Dan Identifikasi Bakteri Asam Laktat Dari Makanan Tradisional Suku Rejang Di Provinsi Bengkulu: “Lemea.” Alotrop. 2020;4(1):25–32. doi: 10.33369/atp. v4i1. 13705

Mastuti S. Potensi Bakteriosin pada Bakteri Asam Laktat terhadap Staphylococcus aureus dan Escherichia coli. J Ilm Kesehat Sandi Husada. 2022;11(1):25–30. doi: 10.35816/ jiskh.v11i1.650

Li H, Li L, Chi Y, Tian Q, Zhou T, Han C, et al. Development of a standardized Gram stain procedure for bacteria and inflammatory cells using an automated staining instrument. Microbiologyopen. 2020;9(9):1–10. doi: 10.1002/mbo3.1099

Manalu RT, Bahri S, Melisa, Sarah S. Isolasi dan Karakterisasi Bakteri Asam Laktat asal Feses Manusia sebagai Antibakteri Escherichia coli dan Staphylococcus aureus. Sainstech Farma. 2020;13(1):55–9. doi:

George F, Daniel C, Thomas M, Singer E, Guilbaud A, Tessier FJ, et al. Occurrence and dynamism of lactic acid bacteria in distinct ecological niches: A multifaceted functional health perspective. Front Microbiol. 2018;9(NOV):1–15. doi: 10.3389/ fmicb. 2018.02899

Mokoena MP. Lactic acid bacteria and their bacteriocins: Classification, biosynthesis and applications against uropathogens: A mini-review. Molecules. 2017;22(8). doi: 10.3390/ molecules22081255

Nofiani R, Ardiningsih P, Adhitiyawarman, Sarwiyati. Characteristics of Lactic Acid Bacteria isolated from traditional fermented fish. Biodiversitas. 2022;23(11):5662–9. doi: 10.13057/biodiv/d231116

Joni LS, Erina E, Abrar M. Total Bakteri Asam Laktat (BAL) pada Feses Rusa Sambar (Cervus unicolor) di Taman Rusa Aceh Besar (The Total of Lactic Acid Bacteria (LAB) on Feces of Sambar Deer (Cervus unicolor) in Taman Rusa Aceh Besar. J Ilm Mhs Vet. 2018;2(2):77–85. doi: 10.21157/jim% 20vet..v2i2.6782

Anindita NS. Isolasi Dan Identifikasi Fenotipik Bakteri Asam Laktat (BAL) Indigenous Asal Air Susu Ibu (ASI). J Teknol Pangan. 2022;5(1):18–23. doi: 10.14710/ jtp.2021.22289

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Published

2024-05-09

How to Cite

Hasbi, N., Rosyunita, Rahim, A. R. ., Ayunda, R. D. ., Parwata, W. S. S. ., Farras, A. ., Raihan, A. F. ., & Billah, M. A. . (2024). Isolasi bakteri asam laktat asal feses bayi dan potensinya dalam menghambat pertumbuhan Escherichia coli. Jurnal Kedokteran Universitas Palangka Raya, 12(1). https://doi.org/10.37304/jkupr.v12i1.12852

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