Aktivitas antibakteri propolis madu putih sumbawa terhadap pertumbuhan Staphylococcus epidermidis, Staphylococcus aureus dan Escherichia coli

Authors

  • Eustachius Hagni Wardoyo Departemen Mikrobiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Rosyunita Departemen Mikrobiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Adelia Riezka Rahim Departemen Mikrobiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Ida Ayu Arnawati Program Studi Pendidikan Dokter, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Saskia Safarina Haza Program Studi Pendidikan Dokter, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Lale Nandhita Hulfifa Program Studi Pendidikan Dokter, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Abiel Dwi Cahya Firdaus Alamsyah Program Studi Pendidikan Dokter, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • I Komang Satya Validika Program Studi Pendidikan Dokter, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram
  • Nurmi Hasbi Departemen Mikrobiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Mataram

Keywords:

antibacterial, Escherichia coli, Staphylococus aureus, Staphylococcus epidermidis, white honey propolis

Abstract

Silent pandemic is a health issue related to antimicrobial resistance. Currently, deaths due to antimicrobial resistance in the world reach 700 thousand people per year and it is predicted that by 2050 it could reach 10 million people. So exploring new alternative sources is a must. One ingredient that is often used as an infection medicine and health supplement is honey propolis. Sumbawa white honey propolis is a biological product that has not yet been widely explored for its potential as an antibacterial. The research aims to determine the phytochemical content and antibacterial potential of Sumbawa white honey propolis against Staphylococcus epidermidis, Staphylococcus aureus and Escherchia coli. This research is a true experiment research with a post-test only control group design. Propolis is extracted by maceration with propylene glycol as a solvent. The concentrations of propolis extract used are 100%, 75% and 50%. Chloramphenicol positive control and 10% DMSO negative control. The antibacterial test was detected in five replications on Mueller Hinton Agar media using the disk diffusion method by measuring the diameter of the inhibition zone. Data were analyzed statistically using the OneWay Anova test. Sumbawa white honey propolis contains flavonoids, tannins and triterpenoids. The results showed that propolis was unable to inhibit gram-negative E. coli bacteria with an inhibition zone diameter of 0 mm. Different results in gram positives, Sumbawa white honey propolis was able to inhibit the growth of S. epidermidis and S. aureus. The results showed that propolis was unable to inhibit gram-negative E. coli bacteria with an inhibition zone diameter of 0 mm. Different results in gram positives, Sumbawa white honey propolis was able to inhibit the growth of S. epidermidis and S. aureus. However, Sumbawa white honey propolis against both gram-positive bacteria was in the weak category compared to the positive control. This is because all the Sumbawa white honey propolis concentration series have an inhibitory zone diameter of ≤ 5 mm.

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Published

2024-06-02

How to Cite

Wardoyo, E. H. ., Rosyunita, Rahim, A. R. ., Arnawati, I. A. ., Haza, S. S. ., Hulfifa, L. N. ., Alamsyah, A. D. C. F. ., Validika, I. K. S. ., & Hasbi, N. . (2024). Aktivitas antibakteri propolis madu putih sumbawa terhadap pertumbuhan Staphylococcus epidermidis, Staphylococcus aureus dan Escherichia coli . Jurnal Kedokteran Universitas Palangka Raya, 12(1). Retrieved from https://e-journal.upr.ac.id/index.php/JK/article/view/12853

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Vol. 12 No. 1 (2024): Jurnal Kedokteran Universitas Palangka Raya

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